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Last updated: 2020/8/2

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Retinoblastoma Gene Protein monoclonal antibody, clone 13A10 

  • Catalog # : MAB22783
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human retinoblastoma gene protein.
  • Immunogen:
  • Recombinant protein corresponding to N terminus of human retinoblastoma gene protein.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • Human retinoblastoma gene protein (105 kD).
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:25-1:50)br>Western Blot (1:250)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In tissue culture supernatant (15 mM sodium azide).
  • Storage Instruction:
  • Store at 4°C. Prepare working dilutions on the day of use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Recommended positive control for immunohistochemistry is tonsil and for western blotting is WI38 cell line.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Note intense nuclear staining of epithelial cells.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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