Retinoblastoma Gene Protein monoclonal antibody, clone 13A10
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Note intense nuclear staining of epithelial cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human retinoblastoma gene protein.
Immunogen
Recombinant protein corresponding to N terminus of human retinoblastoma gene protein.
Host
Mouse
Reactivity
Human
Specificity
Human retinoblastoma gene protein (105 kD).
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:25-1:50)
Western Blot (1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (15 mM sodium azide).
Storage Instruction
Store at 4°C. Prepare working dilutions on the day of use.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Recommended positive control for immunohistochemistry is tonsil and for western blotting is WI38 cell line. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Note intense nuclear staining of epithelial cells.
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