UCHL1 monoclonal antibody, clone 10A1
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human UCHL1.
Immunogen
Recombinant fusion protein corresponding to full length human UCHL1.
Host
Mouse
Reactivity
Human
Specificity
Human protein gene product 9.5.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:40 for 30 minutes at 25°C)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not freeze. Return to 2-8°C immediately after use. Do not use after expiration date indicated on the vial label.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Recommended positive control tissue is small bowel where enteric ganglion cells stain.
Recommended negative control tissue is tonsil where the lymphoid cells are negative. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon. Note the staining in the neuronal elements and the ganglia of the longitudinal and circular smooth muscle. -
Gene Info — UCHL1
Entrez GeneID
7345Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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