MKI67 monoclonal antibody, clone MM1
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of diffuse large B cell lymphoma. Note the intense nuclear staining in proliferating B cells.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human MKI67.
Immunogen
Recombinant fusion protein corresponding to a 1086 bp Ki67 motif-containing cDNA fragment.
Host
Mouse
Reactivity
Human
Specificity
Human Ki67 nuclear antigen expressed in all proliferating cells during late G1, S, M and G2 phases of the cell cycle.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 for 30 minutes at 25°C)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not freeze. Return to 2-8°C immediately after use. Do not use after expiration date indicated on the vial label.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Recommended positive control tissue is tonsil.
Recommended negative control tissue is cerebellum. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of diffuse large B cell lymphoma. Note the intense nuclear staining in proliferating B cells. -
Gene Info — MKI67
Entrez GeneID
4288Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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Interactome
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Disease
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