ANO1 monoclonal antibody, clone K9
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ANO1.
Immunogen
Recombinant protein corresponding to part of the C-terminus of the cytoplasmic domain of human ANO1.
Host
Mouse
Reactivity
Human
Specificity
Human DOG-1.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 for 30 minutes at 25°C)
The optimal working dilution should be determined by the end user.
The use of PBS based diluents may result in increased background staining.Storage Buffer
In tissue culture supernatant (15 mM sodium azide).
Storage Instruction
Store at 4°C. Do not freeze. Return to 2-8°C immediately after use. Do not use after expiration date indicated on the vial label.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Recommended positive control tissue is gastrointestinal stromal tumor (GIST).
Recommended negative control tissue is cerebellum. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon, gastrointestinal stromal tumor. Note membrane staining of neoplastic cells. -
Gene Info — ANO1
Entrez GeneID
55107Gene Name
ANO1
Gene Alias
FLJ10261, ORAOV2, TAOS2, TMEM16A
Gene Description
anoctamin 1, calcium activated chloride channel
Omim ID
610108Gene Ontology
HyperlinkGene Summary
O
Other Designations
membrane protein|oral cancer overexpressed 2|transmembrane protein 16A|transmembrane protein 16A (eight membrane-spanning domains)|tumor amplified and overexpressed sequence 2
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