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AURKA monoclonal antibody, clone JLM28 

  • Catalog # : MAB22773
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human AURKA.
  • Immunogen:
  • Recombinant fusion protein corresponding to 131 amino acid region at N-terminus of human AURKA.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • Human Aurora Kinase 2.
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In tissue culture supernatant (15 mM sodium azide).
  • Storage Instruction:
  • Store at 4°C. Prepare working dilutions on the day of use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Recommended positive control for immunohistochemistry is tonsil and for western blotting is HeLa cell line.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of HeLa cell line. Note nuclear staining of a proportion of cells.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 6790
  • Gene Name:
  • AURKA
  • Gene Alias:
  • AIK,ARK1,AURA,AURORA2,BTAK,MGC34538,STK15,STK6,STK7
  • Gene Description:
  • aurora kinase A
  • Gene Summary:
  • The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • IPL1-related kinase,OTTHUMP00000031340,OTTHUMP00000031342,OTTHUMP00000031343,OTTHUMP00000031344,OTTHUMP00000031345,OTTHUMP00000166071,aurora-A,aurora-related kinase 1,aurora/IPL1-like kinase,breast-tumor-amplified kinase,serine/threonine kinase 15,serine/
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