Mouse Anti-Human IgM (Fc5u) secondary antibody, clone 22G1.H8
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: human IgM, Lane 2: human IgG, Lane 3: human Fc, Lane 4: human Fab, Lane 5: human Fab2, and Lane 6: human IgM Fc5mu. Load: 50 ng whole molecule per lane. Primary antibody: at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase anti-mouse secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicated/Observed size: 78 kDa, 78 kDa for human IgM.
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Specification
Product Description
Mouse monoclonal antibody raised against human IgM Fc5mu fragment.
Immunogen
Human IgM Fc5mu fragment.
Host
Mouse
Reactivity
Human
Specificity
IgM Fc5mu. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, human IgM, human serum and human Fc5mu. No reaction was observed against human IgG, human IgA, human Fc, human Fab or human Fab2.
Form
Liquid
Conjugation
Unconjugated
Purification
This antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads.
Isotype
IgG2b, kappa
Recommend Usage
ELISA (1:1000-1:10000)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
Storage Instruction
Store vial at -20°C prior to opening. For long term storage, aliquot contents and freeze at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: human IgM, Lane 2: human IgG, Lane 3: human Fc, Lane 4: human Fab, Lane 5: human Fab2, and Lane 6: human IgM Fc5mu. Load: 50 ng whole molecule per lane. Primary antibody: at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase anti-mouse secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicated/Observed size: 78 kDa, 78 kDa for human IgM.Enzyme-linked Immunoabsorbent Assay
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