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Mouse Anti-Human IgG F(c) secondary antibody, clone 3D8.H7.D4 

  • Catalog # : MAB22760
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human IgG F(c) fragment.
  • Immunogen:
  • Human IgG F(c) fragment.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, human IgG, human serum and human F(c). No reaction was observed against human F(ab).
  • Form:
  • Liquid
  • Conjugation:
  • Unconjugated
  • Purification:
  • This antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads.
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:1000-1:10000)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at -20°C prior to opening. For long term storage, aliquot contents and freeze at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: human IgG, Lane 2: human Fc, Lane 3: human Fab, Lane 4: human Fab2, Lane 5: human IgM, and Lane 6: human IgM Fc5mu. Load: 50 ng per lane. Primary antibody: at 1:1000 for overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicted MW: ~28 kDa.Observed MW: ~33 kDa for human Fc.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
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