Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Biotin)
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with Fig (A) purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Biotin) (1:20K), Fig (B) coated purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Biotin). followed by Streptavidin-HRP.
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Specification
Product Description
Mouse monoclonal antibody raised against human IgG F(c).
Immunogen
Unknown.
Host
Mouse
Theoretical MW (kDa)
150
Reactivity
Human
Specificity
The antibody reacts with human, rhesus, cynomolgus, chimpanzee, african green monkey, mangabey, pigtail IgG F(c).
Form
Liquid
Conjugation
Biotin
Purification
Precipitation and/or chromatography
Purity
>= 90% purity by HPLC and/or SDS-PAGE
Isotype
IgG2b, kappa
Recommend Usage
ELISA (1:10000-1:20000)
Flow Cytometry (<= 1 ug/106cells): suggested use of the reagent is in a final volume of 100 uL
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with Fig (A) purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Biotin) (1:20K), Fig (B) coated purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Biotin). followed by Streptavidin-HRP.Flow Cytometry
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