Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Alkaline Phosphatase)
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Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Alkaline Phosphatase).
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Specification
Product Description
Mouse monoclonal antibody raised against human IgG F(c).
Immunogen
Unknown.
Host
Mouse
Theoretical MW (kDa)
150
Reactivity
Human
Specificity
The antibody reacts with human, rhesus, cynomolgus, chimpanzee, african green monkey, mangabey, pigtail IgG F(c).
Form
Liquid
Conjugation
Alkaline Phosphatase
Purification
Precipitation and/or chromatography
Purity
>= 90% purity by HPLC and/or SDS-PAGE
Isotype
IgG2b, kappa
Recommend Usage
ELISA (1:2000-1:8000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris, 1 mM MgCl2, pH 8.0 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Alkaline Phosphatase).
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