Mouse Anti-Human IgG F(c) secondary antibody, clone H2
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Cat # MAB22730) followed by Goat Anti-Mouse IgG (H+L)/Multi Species serum proteins ads (Biotin) and Streptavidin-HRP.
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Specification
Product Description
Mouse monoclonal antibody raised against human IgG F(c).
Immunogen
Unknown.
Host
Mouse
Theoretical MW (kDa)
150
Reactivity
Human
Specificity
The antibody reacts with human, rhesus, cynomolgus, chimpanzee, african green monkey, mangabey, pigtail IgG F(c).
Form
Liquid
Purification
Precipitation and/or chromatography
Purity
>= 90% purity by HPLC and/or SDS-PAGE
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry (<= 1 ug/106cells): suggested use of the reagent is in a final volume of 100 uL
The optimal working dilution should be determined by the end user.Storage Buffer
In BBS, pH 8.2.
Storage Instruction
Store at 4°C.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG F(c) secondary antibody, clone H2 (Cat # MAB22730) followed by Goat Anti-Mouse IgG (H+L)/Multi Species serum proteins ads (Biotin) and Streptavidin-HRP.Flow Cytometry
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