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Last updated: 2020/5/24

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SARS-CoV N monoclonal antibody, clone 3851 

  • Catalog # : MAB22372
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SARS-CoV N.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-49 of SARS-CoV Nucleoprotein.
  • Host:
  • Mouse
  • Reactivity:
  • Viruses
  • Specificity:
  • Specific for the nucleoprotein of the SARS virus. This antibody cross react with native SARS virus and with recombinant Covid-19 NP. They do not cross react with native MERS virus.
  • Form:
  • Liquid
  • Purification:
  • Protein A chromoatography or sequential differential precipitations.
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.01 M PBS, pH 7.2 (0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot with human recombinant protein. Loading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV N monoclonal antibody, clone 3851 (Cat # MAB22372), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL.
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and this antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 2000 ng/mL.
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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