JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cervix cancer. Phospho-JAK2 (Y1007/Y1008) was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 minutes. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/mL JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10 overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Immunocytochemistry
Immunocytochemical staining of Jurkat cells treated with Pervanadate using JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10. Phospho-JAK2 (Y1007/Y1008) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 minutes. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10 overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human JAK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y1007/Y1008 of human JAK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:30)
Immunocytochemistry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cervix cancer. Phospho-JAK2 (Y1007/Y1008) was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 minutes. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/mL JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10 overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.Immunocytochemistry
Immunocytochemical staining of Jurkat cells treated with Pervanadate using JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10. Phospho-JAK2 (Y1007/Y1008) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 minutes. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with JAK2 (phospho Y1007/Y1008) monoclonal antibody, clone IFE-10 overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.Immunoprecipitation
Flow Cytometry
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Gene Info — JAK2
Entrez GeneID
3717Protein Accession#
O60674Gene Name
JAK2
Gene Alias
JTK10
Gene Description
Janus kinase 2 (a protein tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq
Other Designations
Janus kinase 2|OTTHUMP00000021024|OTTHUMP00000043260|tyrosine-protein kinase JAK2
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Interactome
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Pathway
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Disease
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