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Last updated: 2022/5/15

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AP2A1 monoclonal antibody, clone AFDO-1 

  • Catalog # : MAB22286
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human AP2A1.
  • Immunogen:
  • A synthetic peptide corresponding to human AP2A1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • The antibody reacts with human AP2A1, in native form and recombinant. Superfamily members of AP2A1 are not reactive to this antibody.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term storage. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A-431 cell lysate.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 160
  • Gene Name:
  • AP2A1
  • Gene Alias:
  • ADTAA,AP2-ALPHA,CLAPA1
  • Gene Description:
  • adaptor-related protein complex 2, alpha 1 subunit
  • Gene Summary:
  • This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
  • Other Designations:
  • 100 kDa coated vesicle protein A,adaptin, alpha A,clathrin-associated/assembly/adaptor protein, large, alpha 1
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