Rabbit monoclonal antibody raised against synthetic peptide of human HIST1H1C.
Immunogen:
A synthetic peptide corresponding to human HIST1H1C.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
The antibody reacts with human, mouse, rat HIST1H1C, in native form and recombinant. Superfamily members of HIST1H1C are not reactive to this antibody.
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (1:50-1:200) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse colon tissue.
Immunocytochemistry
Immunocytochemistry staining of Hela cells (green), the nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations:
H1 histone family, member 2,OTTHUMP00000017749,histone 1, H1c,histone H1d