DNM1 monoclonal antibody, clone ACHH-4
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human DNM1.
Immunogen
A synthetic peptide corresponding to human DNM1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
The antibody reacts with human, mouse DNM1, in native form and recombinant. Superfamily members of DNM1 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: SH-SY5Y cell lysate; Lane 2: NIH/3T3 cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
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Gene Info — DNM1
Entrez GeneID
1759Protein Accession#
Q05193Gene Name
DNM1
Gene Alias
DNM
Gene Description
dynamin 1
Omim ID
602377Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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