NCK1 monoclonal antibody, clone AFEG-14
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human NCK1.
Immunogen
A synthetic peptide corresponding to human NCK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat NCK1, in native form and recombinant. Superfamily members of NCK1 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of HeLa cell lysate. -
Gene Info — NCK1
Entrez GeneID
4690Protein Accession#
P16333Gene Name
NCK1
Gene Alias
MGC12668, NCK, NCKalpha
Gene Description
NCK adaptor protein 1
Omim ID
600508Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq
Other Designations
NCK tyrosine kinase|SH2/SH3 adaptor protein NCK-alpha|melanoma NCK protein|non-catalytic region of tyrosine kinase
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Interactome
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Pathway
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