NRF1 monoclonal antibody, clone ADGI-14
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of NRF1 in HeLa cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human NRF1.
Immunogen
A synthetic peptide corresponding to human NRF1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human NRF1, in native form and recombinant. Superfamily members of NRF1 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of NRF1 in HeLa cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — NRF1
Entrez GeneID
4899Protein Accession#
Q16656Gene Name
NRF1
Gene Alias
ALPHA-PAL
Gene Description
nuclear respiratory factor 1
Omim ID
600879Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq
Other Designations
OTTHUMP00000184912|alpha palindromic-binding protein
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Interactome
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Disease
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