TFAM monoclonal antibody, clone ADHC-20
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human TFAM.
Immunogen
A synthetic peptide corresponding to human TFAM.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human TFAM, in native form and recombinant. Superfamily members of TFAM are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of TFAM in MCF-7 cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — TFAM
Entrez GeneID
7019Protein Accession#
Q00059Gene Name
TFAM
Gene Alias
MtTF1, TCF6, TCF6L1, TCF6L2, TCF6L3, mtTFA
Gene Description
transcription factor A, mitochondrial
Omim ID
600438Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq
Other Designations
OTTHUMP00000019633|Transcription factor 6-like 2 (mitochondrial transcription factor)|mitochondrial transcription factor A
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