ADORA1 monoclonal antibody, clone ACDG-1
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ADORA1.
Immunogen
A synthetic peptide corresponding to human ADORA1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human ADORA1, in native form and recombinant. Superfamily members of ADORA1 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of ADORA1 in A-549 cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Immunoprecipitation analysis of ADORA1 expression in A-549 cell lysate. Rabbit IgG was coupled to A1R via Co-IP.Flow Cytometry
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Gene Info — ADORA1
Entrez GeneID
134Protein Accession#
P30542Gene Name
ADORA1
Gene Alias
RDC7
Gene Description
adenosine A1 receptor
Omim ID
102775Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000039090|OTTHUMP00000039091
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Interactome
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