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Western blot analysis of Lane 1: human THP-1 cell lysates, Lane 2: human U-937 cell lysates, Lane 3: human HL-60 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse RAW246.7 tissue lysates.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using AIF1 monoclonal antibody, clone AEBB-1. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the AIF1 monoclonal antibody, clone AEBB-1 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunofluorescence staining of paraffin- embedded human spleen tissue using AIF1 monoclonal antibody, clone AEBB-1. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AIF1 monoclonal antibody, clone AEBB-1 at 1/50 dilution for 10 hours at 4°C and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.