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Last updated: 2021/8/8

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AIF1 monoclonal antibody, clone AEBB-1 

  • Catalog # : MAB22142
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human AIF1.
  • Immunogen:
  • A synthetic peptide corresponding to human AIF1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • The antibody reacts with human, mouse, rat AIF1, in native form and recombinant. Superfamily members of AIF1 are not reactive to this antibody.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:100-1:500)
    Immunoprecipitation (1:50)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term storage. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: human THP-1 cell lysates, Lane 2: human U-937 cell lysates, Lane 3: human HL-60 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse RAW246.7 tissue lysates.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using AIF1 monoclonal antibody, clone AEBB-1. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the AIF1 monoclonal antibody, clone AEBB-1 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of paraffin- embedded human spleen tissue using AIF1 monoclonal antibody, clone AEBB-1. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AIF1 monoclonal antibody, clone AEBB-1 at 1/50 dilution for 10 hours at 4°C and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunoprecipitation
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 199
  • Gene Name:
  • AIF1
  • Gene Alias:
  • AIF-1,IBA1,IRT-1
  • Gene Description:
  • allograft inflammatory factor 1
  • Gene Summary:
  • This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000029354,OTTHUMP00000029356,interferon gamma responsive transcript,ionized calcium-binding adapter molecule
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