EIF2AK4 monoclonal antibody, clone ACAA-5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemistry analysis of EIF2AK4 in A-549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with Cat # MAB22121 at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human EIF2AK4.
Immunogen
A synthetic peptide corresponding to human EIF2AK4.
Host
Rabbit
Reactivity
Human
Specificity
The antibody reacts with human EIF2AK4, in native form and recombinant. Superfamily members of EIF2AK4 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemistry analysis of EIF2AK4 in A-549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunofluorescence
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with Cat # MAB22121 at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody). -
Gene Info — EIF2AK4
Entrez GeneID
440275Protein Accession#
Q9P2K8Gene Name
EIF2AK4
Gene Alias
GCN2, KIAA1338
Gene Description
eukaryotic translation initiation factor 2 alpha kinase 4
Omim ID
609280Gene Ontology
HyperlinkGene Summary
EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM
Other Designations
GCN2 eIF2alpha kinase
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