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EIF2AK4 monoclonal antibody, clone ACAA-5 

  • Catalog # : MAB22121
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human EIF2AK4.
  • Immunogen:
  • A synthetic peptide corresponding to human EIF2AK4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • The antibody reacts with human EIF2AK4, in native form and recombinant. Superfamily members of EIF2AK4 are not reactive to this antibody.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term storage. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HeLa cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil. Counter stained with hematoxylin.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry analysis of EIF2AK4 in A-549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunofluorescence
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells with Cat # MAB22121 at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Gene Name:
  • EIF2AK4
  • Gene Alias:
  • GCN2,KIAA1338
  • Gene Description:
  • eukaryotic translation initiation factor 2 alpha kinase 4
  • Gene Summary:
  • EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM
  • Other Designations:
  • GCN2 eIF2alpha kinase
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