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Last updated: 2020/2/23

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Histone H4 monoclonal antibody, clone RM212 

  • Catalog # : MAB22117
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against partial synthetic protein of human Histone H4.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human Histone H4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Multiplex (0.1-0.5 ug/mL)
    Western Blot (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 50% Glycerol/PBS (1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) A375, (2) HEK293, (3) HeLa, (4) SK-MEL-2 whole cell lysates.
  • Multiplex
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of naive HepG2 cells.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Multiplex
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 8370
  • Gene Name:
  • HIST2H4A
  • Gene Alias:
  • FO108,H4,H4/n,H4F2,H4FN,HIST2H4
  • Gene Description:
  • histone cluster 2, H4a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq
  • Other Designations:
  • H4 histone family, member N,H4 histone, family 2,OTTHUMP00000013906,OTTHUMP00000194862,OTTHUMP00000194863,histone 2, H4a,histone IV, family 2
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