CKB monoclonal antibody, clone AFOC-3
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of SH-SY5Y cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human CKB.
Immunogen
A synthetic peptide corresponding to human CKB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat CKB, in native form and recombinant. Superfamily members of CKB are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Immunoprecipitation (1:50)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of SH-SY5Y cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — CKB
Entrez GeneID
1152Protein Accession#
P12277Gene Name
CKB
Gene Alias
B-CK, CKBB
Gene Description
creatine kinase, brain
Omim ID
123280Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq
Other Designations
brain creatine kinase|creatine kinase B-chain|creatine kinase-B
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Interactome
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Pathway
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Disease
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