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Last updated: 2020/8/2

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VPS35 monoclonal antibody, clone AECH-22 

  • Catalog # : MAB22111
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic protein of human VPS35.
  • Immunogen:
  • A synthetic peptide corresponding to human VPS35.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody reacts with human, mouse, rat VPS35, in native form and recombinant. Superfamily members of VPS35 are not reactive to antibody.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
    Immunofluorescence (1:50-200)
    Immunocytochemistry (1:50-200)
    Western Blot (1:1000-5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) HeLa cell lysate, (2) Mouse kidney lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • VPS35
  • Gene Alias:
  • DKFZp434E1211,DKFZp434P1672,FLJ10752,FLJ13588,FLJ20388,MEM3
  • Gene Description:
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • Gene Summary:
  • This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
  • Other Designations:
  • maternal-embryonic 3,vacuolar protein sorting 35
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