IQGAP1 monoclonal antibody, clone ADGG-9
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human IQGAP1.
Immunogen
A synthetic peptide corresponding to human IQGAP1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody reacts with human, mouse IQGAP1, in native form and recombinant. Superfamily members of IQGAP1 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — IQGAP1
Entrez GeneID
8826Protein Accession#
P46940Gene Name
IQGAP1
Gene Alias
HUMORFA01, KIAA0051, SAR1, p195
Gene Description
IQ motif containing GTPase activating protein 1
Omim ID
603379Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq
Other Designations
RasGAP-like with IQ motifs
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Interactome
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Pathway
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