HPRT1 monoclonal antibody, clone ADAB-8
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human HPRT1.
Immunogen
A synthetic peptide corresponding to human HPRT1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat HPRT1, in native form and recombinant. Superfamily members of HPRT1 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunoprecipitation (1:50)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of A549 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — HPRT1
Entrez GeneID
3251Protein Accession#
P00492Gene Name
HPRT1
Gene Alias
HGPRT, HPRT
Gene Description
hypoxanthine phosphoribosyltransferase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout
Other Designations
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Interactome
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Pathway
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Disease
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