WNT5A monoclonal antibody, clone ABEE-23
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human WNT5A.
Immunogen
A synthetic peptide corresponding to human WNT5A.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human WNT5A, in native form and recombinant. Superfamily members of WNT5A are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of HeLa cell lysate.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — WNT5A
Entrez GeneID
7474Protein Accession#
P41221Gene Name
WNT5A
Gene Alias
hWNT5A
Gene Description
wingless-type MMTV integration site family, member 5A
Omim ID
164975Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq
Other Designations
WNT-5A protein
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Interactome
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Pathway
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Disease
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