IgM heavy chain monoclonal antibody, clone RM121 (Biotin)
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More Files
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Enzyme-linked Immunoabsorbent Assay
A titer ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011). The plate was coated with different amounts of human IgM. A serial dilution of Cat# MAB22011 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgM heavy chain.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgM.
Reactivity
Human
Specificity
This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (50-200 ng/well (for Capture); 0.05-0.2 ug/mL (for Detection))
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
Immunocytochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011). The plate was coated with different amounts of human IgM. A serial dilution of Cat# MAB22011 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using IgM heavy chain monoclonal antibody, clone RM121 (Biotin) (Cat# MAB22011) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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