ALK monoclonal antibody, clone RM361
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of NSCLC cell line H2228 expressing EML4-ALK variant 3.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ALK.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human ALK.
Reactivity
Human
Specificity
This antibody reacts to human total ALK protein including ALK fusion proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1000)
Western Blot (1:1000-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of NSCLC cell line H2228 expressing EML4-ALK variant 3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative). -
Gene Info — ALK
Entrez GeneID
238Gene Name
ALK
Gene Alias
CD246, Ki-1, TFG/ALK
Gene Description
anaplastic lymphoma receptor tyrosine kinase
Omim ID
105590Gene Ontology
HyperlinkGene Summary
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
Other Designations
ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma kinase (Ki-1)|anaplastic lymphoma kinase Ki-1
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Interactome
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Disease
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