AKT1 (E17K mutant) monoclonal antibody, clone RM336
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) 293T transfected with a DNA construct encoding the Akt E17K mutant, and (2) untransfected 293T.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of 293T cells transfected with a DNA construct encoding the Akt E17K mutation or Histone H3.3.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against AKT1 (E17K mutant).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to AKT1 (E17K mutant).
Specificity
This antibody reacts to the Akt E17K mutant. No cross reactivity with wild type Akt.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.05 ug/mL-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.2 ug/mL-1 ug/mL)
Western Blot (0.1 ug/mL-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) 293T transfected with a DNA construct encoding the Akt E17K mutant, and (2) untransfected 293T.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of 293T cells transfected with a DNA construct encoding the Akt E17K mutation or Histone H3.3.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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