Histone H3 (crotonyl K9) monoclonal antibody, clone RM339
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic crotonyl peptide corresponding to residues surrounding Lys9 of human histone H3.
Reactivity
Human
Specificity
This antibody reacts to Histone H3 crotonylated at Lysine 9 (K9cr). No cross reactivity with other crotonylated or acetylated Lysines in histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Dot Blot (0.5 ug/mL-2 ug/mL)
Multiplex (0.05 ug/mL-0.5 ug/mL)
Western Blot (1 ug/mL-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of crotonyl-Histone H3 (K9).Dot Blot (Peptide)
Dot Blot (Peptide) reacts specifically to Histone H3 crotonylated at Lysine 9, and it cross-reactivity with different peptides.Multiplex
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