Histone H3 (trimethyl K4) monoclonal antibody, clone RM340
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More Files
- More Functions
ChIP
ChIP analysis of HEK-293 chromatin (~1x106cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the ChIP panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic trimethyl peptide corresponding to residues surrounding Lys4 of human histone H3.
Reactivity
Human
Specificity
This antibody reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ChIP (1 ug/mL-5 ug/mL)
ELISA (1 ug/mL-10 ug/mL)
Multiplex (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP analysis of HEK-293 chromatin (~1x106cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the ChIP panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified).Multiplex
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