KDEL monoclonal antibody, clone 10C3
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) Grp78 (BiP) recombinant protein (2) RK-13 (3) mouse liver microsomes (4) rat liver microsomes, and (5) heat-shocked HeLa cell lysate.
Immunohistochemistry
Immunohistochemical staining of human spleen at 10 ug/mL.
Immunofluorescence
Immunofluorescent staining of ER in mouse C2C12 myoblasts transfected with wild-type mouse ADAM12 with MAB2193 at 10 ug/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of KDEL.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 649-654 of rat Hspa5.
Host
Mouse
Specificity
This antibody recognizes proteins containing the KDEL sequence.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (5-10 ug/mL)
Western Blot (4 ug/mL) (Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) Grp78 (BiP) recombinant protein (2) RK-13 (3) mouse liver microsomes (4) rat liver microsomes, and (5) heat-shocked HeLa cell lysate.Immunohistochemistry
Immunohistochemical staining of human spleen at 10 ug/mL.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of ER in mouse C2C12 myoblasts transfected with wild-type mouse ADAM12 with MAB2193 at 10 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Hspa5
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