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Last updated: 2021/6/13
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KDEL monoclonal antibody, clone 10C3 

  • Catalog # : MAB2193
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of KDEL.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 649-654 of rat Hspa5.
  • Host:
  • Mouse
  • Specificity:
  • This antibody recognizes proteins containing the KDEL sequence.
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunohistochemistry (5-10 ug/mL)
    Western Blot (4 ug/mL) (Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) Grp78 (BiP) recombinant protein (2) RK-13 (3) mouse liver microsomes (4) rat liver microsomes, and (5) heat-shocked HeLa cell lysate.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human spleen at 10 ug/mL.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of ER in mouse C2C12 myoblasts transfected with wild-type mouse ADAM12 with MAB2193 at 10 ug/mL.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • Hspa5
  • Gene Alias:
  • BIP
  • Gene Description:
  • heat shock protein 5
  • Other Designations:
  • heat shock 70kD protein 5,heat shock 70kDa protein 5 (glucose-regulated protein)
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