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Last updated: 2019/8/18

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Histone H3 (trimethyl K27) monoclonal antibody 

  • Catalog # : MAB21772
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of Histone H3.
  • Immunogen:
  • A synthetic methylated peptide corresponding to residues surrounding K27 of Histone H3.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP (1:20-1:100)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Immunoprecipitation (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of C6 cells with Histone H3 (trimethyl K27) monoclonal antibody (Cat # MAB21772).
  • Immunoprecipitation
  • Application Image
  • ChIP
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8290
  • Gene Name:
  • HIST3H3
  • Gene Alias:
  • H3.4,H3/g,H3FT,H3t,MGC126886,MGC126888
  • Gene Description:
  • histone cluster 3, H3
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member T,OTTHUMP00000037945,histone 3, H3
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