IP6K2 monoclonal antibody, clone 4F10
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More Files
- More Functions
Western Blot (Cell lysate)
Effect of gamma-irradiation and IFN-beta on IP6K2 protein levels.
(a) NIH:OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 (white bars) or 2 Gy (gray bars) irradiation and replated in complete medium.
At 8 and 16 h after plating, cells were lysed and subject to Western blot analysis using IP6K2 monoclonal antibody, clone 4F10 (Cat # MAB2177).
To control for loading, blots were probed with anti-actin antibody; bands were digitized and quantified.
IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1.
Purified rIP6K2 was included as a positive control (lane 1).
Numbers to right of blots indicate MW markers.
Only relevant portion of the blots are shown.
(b) NIH-OVAR-3 cells were grown in the presence of 200 U/ml IFN-beta for 0-48 h and then subject to Western blot analysis and quantified as above.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant IP6K2.
Immunogen
Recombinant protein corresponding to full length human IP6K2.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes human IP6K2.
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Effect of gamma-irradiation and IFN-beta on IP6K2 protein levels.
(a) NIH:OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 (white bars) or 2 Gy (gray bars) irradiation and replated in complete medium.
At 8 and 16 h after plating, cells were lysed and subject to Western blot analysis using IP6K2 monoclonal antibody, clone 4F10 (Cat # MAB2177).
To control for loading, blots were probed with anti-actin antibody; bands were digitized and quantified.
IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1.
Purified rIP6K2 was included as a positive control (lane 1).
Numbers to right of blots indicate MW markers.
Only relevant portion of the blots are shown.
(b) NIH-OVAR-3 cells were grown in the presence of 200 U/ml IFN-beta for 0-48 h and then subject to Western blot analysis and quantified as above. -
Gene Info — IP6K2
Entrez GeneID
51447Gene Name
IP6K2
Gene Alias
IHPK2, PiUS
Gene Description
inositol hexakisphosphate kinase 2
Omim ID
606992Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 and affect the growth suppressive and apoptotic activities of interferon-beta in some ovarian cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
ATP:1D-myo-inositol-hexakisphosphate phosphotransferase|OTTHUMP00000164824|Pi uptake stimulator|inositol hexaphosphate kinase 2|insP6 kinase 2
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