Effect of gamma-irradiation and IFN-beta on IP6K2 protein levels. (a) NIH:OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 (white bars) or 2 Gy (gray bars) irradiation and replated in complete medium. At 8 and 16 h after plating, cells were lysed and subject to Western blot analysis using IP6K2 monoclonal antibody, clone 4F10 (Cat # MAB2177). To control for loading, blots were probed with anti-actin antibody; bands were digitized and quantified. IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1. Purified rIP6K2 was included as a positive control (lane 1). Numbers to right of blots indicate MW markers. Only relevant portion of the blots are shown. (b) NIH-OVAR-3 cells were grown in the presence of 200 U/ml IFN-beta for 0-48 h and then subject to Western blot analysis and quantified as above.
This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 and affect the growth suppressive and apoptotic activities of interferon-beta in some ovarian cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq