MARCKS (phospho S167/S170) monoclonal antibody, clone C9
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of NIH3T3 cell lysate with MARCKS (phospho S167/S170) monoclonal antibody, clone C9 (Cat # MAB21666). Lane 1: untreated or Lane 2: treated with TPA.
Flow Cytometry
Flow cytometric analysis of C6 cells with MARCKS (phospho S167/S170) monoclonal antibody, clone C9 (Cat # MAB21666). Secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV+TPA (green).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MARCKS.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S167/S170 of human MARCKS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of NIH3T3 cell lysate with MARCKS (phospho S167/S170) monoclonal antibody, clone C9 (Cat # MAB21666). Lane 1: untreated or Lane 2: treated with TPA.Flow Cytometry
Flow cytometric analysis of C6 cells with MARCKS (phospho S167/S170) monoclonal antibody, clone C9 (Cat # MAB21666). Secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV+TPA (green). -
Gene Info — MARCKS
Entrez GeneID
4082Protein Accession#
P29966Gene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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Interactome
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Pathway
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