CHD4 monoclonal antibody, clone 3D3E9
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CHD4.
Immunogen
Recombinant protein corresponding to amino acids 1-194 of human CHD4.
Host
Mouse
Theoretical MW (kDa)
218
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CHD4-hIgGFc transfected HEK293 cell lysates with CHD4 monoclonal antibody, clone 3D3E9 (Cat # MAB21499).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CHD4 monoclonal antibody, clone 3D3E9 (Cat # MAB21499). -
Gene Info — CHD4
Entrez GeneID
1108Protein Accession#
Q14839Gene Name
CHD4
Gene Alias
DKFZp686E06161, Mi-2b, Mi2-BETA
Gene Description
chromodomain helicase DNA binding protein 4
Omim ID
603277Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq
Other Designations
Mi-2 autoantigen 218 kDa protein
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