EZH1 monoclonal antibody, clone 4B11B4
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EZH1.
Immunogen
Recombinant protein corresponding to amino acids 135-294 of human EZH1.
Host
Mouse
Theoretical MW (kDa)
85.3
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: EZH1-hIgGFc transfected HEK293 cell lysates with EZH1 monoclonal antibody, clone 4B11B4 (Cat # MAB21477).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with EZH1 monoclonal antibody, clone 4B11B4 (Cat # MAB21477).Flow Cytometry
Flow cytometric analysis of HeLa cells with EZH1 monoclonal antibody, clone 4B11B4 (Cat # MAB21477) (Green). Red: Negative Control. -
Gene Info — EZH1
Entrez GeneID
2145Protein Accession#
Q92800Gene Name
EZH1
Gene Alias
KIAA0388
Gene Description
enhancer of zeste homolog 1 (Drosophila)
Omim ID
601674Gene Ontology
HyperlinkGene Summary
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM
Other Designations
enhancer of zeste homolog 1
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Interactome
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Disease
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