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Last updated: 2021/6/13

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PCK2 monoclonal antibody, clone 3D3D9 

  • Catalog # : MAB21474
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human PCK2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 44-175 of human PCK2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 70.7
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:200-1:1000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: PCK2-hIgGFc transfected HEK293 cell lysates with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach carcinoma with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5106
  • Gene Name:
  • PCK2
  • Gene Alias:
  • PEPCK,PEPCK-M,PEPCK2
  • Gene Description:
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Gene Summary:
  • This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164700,PEP carboxykinase,mitochondrial phosphoenolpyruvate carboxykinase 2,phosphoenolpyruvate carboxylase,phosphopyruvate carboxylase
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