RBM9 monoclonal antibody, clone 1E7G10
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human RBM9.
Immunogen
Recombinant protein corresponding to amino acids 1-145 of human RBM9.
Host
Mouse
Theoretical MW (kDa)
41.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: RBM9-hIgGFc transfected HEK293 cell lysates with RBM9 monoclonal antibody, clone 1E7G10 (Cat # MAB21469).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with RBM9 monoclonal antibody, clone 1E7G10 (Cat # MAB21469).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with RBM9 monoclonal antibody, clone 1E7G10 (Cat # MAB21469).Flow Cytometry
Flow cytometric analysis of Jurkat cells with RBM9 monoclonal antibody, clone 1E7G10 (Cat # MAB21469) (Green). Red: Negative Control. -
Gene Info — RBM9
Entrez GeneID
23543Protein Accession#
O43251Gene Name
RBM9
Gene Alias
FOX2, Fox-2, HNRBP2, HRNBP2, RTA, dJ106I20.3, fxh
Gene Description
RNA binding motif protein 9
Gene Ontology
HyperlinkGene Summary
This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028771|fox-1 homologue|hexaribonucleotide binding protein 2|repressor of tamoxifen transcriptional activity
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