FUT3 monoclonal antibody, clone 1F8G5C7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: FUT3-hIgGFc transfected HEK293 cell lysates with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human FUT3.
Immunogen
Recombinant protein corresponding to amino acids 199-361 of human FUT3.
Host
Mouse
Theoretical MW (kDa)
42.1
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: FUT3-hIgGFc transfected HEK293 cell lysates with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462).Flow Cytometry
Flow cytometric analysis of HL-60 cells with FUT3 monoclonal antibody, clone 1F8G5C7 (Cat # MAB21462) (Green). Red: Negative Control. -
Gene Info — FUT3
Entrez GeneID
2525Protein Accession#
P21217Gene Name
FUT3
Gene Alias
CD174, FT3B, FucT-III, LE, Les, MGC131739
Gene Description
fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Omim ID
111100Gene Ontology
HyperlinkGene Summary
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
Lewis FT|alpha-(1,3/1,4)-fucosyltransferase|blood group Lewis alpha-4-fucosyltransferase|fucosyltransferase 3|galactoside 3(4)-L-fucosyltransferase
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Interactome
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Pathway
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Disease
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