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Last updated: 2021/6/20

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TLR9 monoclonal antibody, clone 5B10E8 

  • Catalog # : MAB21457
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human TLR9.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 26-193 of human TLR9.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 116
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: TLR9-hIgGFc transfected HEK293 cell lysates with TLR9 monoclonal antibody, clone 5B10E8 (Cat # MAB21457).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with TLR9 monoclonal antibody, clone 5B10E8 (Cat # MAB21457).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells with TLR9 monoclonal antibody, clone 5B10E8 (Cat # MAB21457) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Gene Name:
  • TLR9
  • Gene Alias:
  • CD289
  • Gene Description:
  • toll-like receptor 9
  • Gene Summary:
  • The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq
  • Other Designations:
  • -
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