BCAM monoclonal antibody, clone 8C4E6
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human BCAM.
Immunogen
Recombinant protein corresponding to amino acids 32-197 of human BCAM.
Host
Mouse
Theoretical MW (kDa)
67.4
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: BCAM-hIgGFc transfected HEK293 cell lysates with BCAM monoclonal antibody, clone 8C4E6 (Cat # MAB21449).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bladder carcinoma with BCAM monoclonal antibody, clone 8C4E6 (Cat # MAB21449).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with BCAM monoclonal antibody, clone 8C4E6 (Cat # MAB21449).Flow Cytometry
Flow cytometric analysis of MOLT4 cells with BCAM monoclonal antibody, clone 8C4E6 (Cat # MAB21449) (Green). Red: Negative Control. -
Gene Info — BCAM
Entrez GeneID
4059Protein Accession#
P50895Gene Name
BCAM
Gene Alias
AU, CD239, LU, MSK19
Gene Description
basal cell adhesion molecule (Lutheran blood group)
Omim ID
111200Gene Ontology
HyperlinkGene Summary
Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Auberger b antigen|B-CAM cell surface glycoprotein|B-cell adhesion molecule|F8/G253 antigen|Lutheran blood group (Auberger b antigen included)|antigen identified by monoclonal antibody F8|basal cell adhesion molecule|basal cell adhesion molecule (Lu and A
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Interactome
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Disease
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