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Last updated: 2021/6/20

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IL5RA monoclonal antibody, clone 5E12F5D6 

  • Catalog # : MAB21428
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human IL5RA.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 21-196 of human IL5RA.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 47.7
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: IL5RA-hIgGFc transfected HEK293 cell lysates with IL5RA monoclonal antibody, clone 5E12F5D6 (Cat # MAB21428).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with IL5RA monoclonal antibody, clone 5E12F5D6 (Cat # MAB21428).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells with IL5RA monoclonal antibody, clone 5E12F5D6 (Cat # MAB21428) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3568
  • Gene Name:
  • IL5RA
  • Gene Alias:
  • CD125,CDw125,HSIL5R3,IL5R,MGC26560
  • Gene Description:
  • interleukin 5 receptor, alpha
  • Gene Summary:
  • The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • CD125 antigen,OTTHUMP00000115257,OTTHUMP00000115373,interleukin 5 receptor type 3,interleukin-5 receptor alpha chain,interleukin-5 receptor alpha subunit
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