JAM2 monoclonal antibody, clone 7E4C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: JAM2-hIgGFc transfected HEK293 cell lysates with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human JAM2.
Immunogen
Recombinant protein corresponding to amino acids 29-238 of human JAM2.
Host
Mouse
Theoretical MW (kDa)
33.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:100)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: JAM2-hIgGFc transfected HEK293 cell lysates with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).Flow Cytometry
Flow cytometric analysis of HL-60 cells with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414) (Green). Red: Negative Control. -
Gene Info — JAM2
Entrez GeneID
58494Protein Accession#
P57087Gene Name
JAM2
Gene Alias
C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM
Gene Description
junctional adhesion molecule 2
Omim ID
606870Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq
Other Designations
JAM-IT/VE-JAM|OTTHUMP00000096100|junctional adhesion molecule B|vascular endothelial junction-associated molecule
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Interactome
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Pathway
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