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Last updated: 2021/6/6

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JAM2 monoclonal antibody, clone 7E4C5 

  • Catalog # : MAB21414
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human JAM2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 29-238 of human JAM2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 33.2
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:100)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: JAM2-hIgGFc transfected HEK293 cell lysates with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells with JAM2 monoclonal antibody, clone 7E4C5 (Cat # MAB21414) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Gene Name:
  • JAM2
  • Gene Alias:
  • C21orf43,CD322,JAM-B,JAMB,PRO245,VE-JAM,VEJAM
  • Gene Description:
  • junctional adhesion molecule 2
  • Gene Summary:
  • Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq
  • Other Designations:
  • JAM-IT/VE-JAM,OTTHUMP00000096100,junctional adhesion molecule B,vascular endothelial junction-associated molecule
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