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Last updated: 2021/6/13

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CD177 monoclonal antibody, clone 2F2C5 

  • Catalog # : MAB21409
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CD177.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 22-161 of human CD177.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 46.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: CD177-hIgGFc transfected HEK293 cell lysates with CD177 monoclonal antibody, clone 2F2C5 (Cat # MAB21409).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with CD177 monoclonal antibody, clone 2F2C5 (Cat # MAB21409).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells with CD177 monoclonal antibody, clone 2F2C5 (Cat # MAB21409) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Gene Name:
  • CD177
  • Gene Alias:
  • HNA2A,NB1,PRV1
  • Gene Description:
  • CD177 molecule
  • Gene Summary:
  • NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM
  • Other Designations:
  • CD177 antigen,cell surface receptor,polycythemia rubra vera 1
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