CD177 monoclonal antibody, clone 2F2C1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CD177-hIgGFc transfected HEK293 cell lysates with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD177.
Immunogen
Recombinant protein corresponding to amino acids 22-161 of human CD177.
Host
Mouse
Theoretical MW (kDa)
46.4
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CD177-hIgGFc transfected HEK293 cell lysates with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408).Flow Cytometry
Flow cytometric analysis of HL-60 cells with CD177 monoclonal antibody, clone 2F2C1 (Cat # MAB21408) (Green). Red: Negative Control. -
Gene Info — CD177
Entrez GeneID
57126Protein Accession#
Q8N6Q3Gene Name
CD177
Gene Alias
HNA2A, NB1, PRV1
Gene Description
CD177 molecule
Omim ID
162860Gene Ontology
HyperlinkGene Summary
NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM
Other Designations
CD177 antigen|cell surface receptor|polycythemia rubra vera 1
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