SIGLEC1 monoclonal antibody, clone 2A4D1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: SIGLEC1-hIgGFc transfected HEK293 cell lysates with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human SIGLEC1.
Immunogen
Recombinant protein corresponding to amino acids 20-197 of human SIGLEC1.
Host
Mouse
Theoretical MW (kDa)
182.6
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: SIGLEC1-hIgGFc transfected HEK293 cell lysates with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407).Flow Cytometry
Flow cytometric analysis of HL-60 cells with SIGLEC1 monoclonal antibody, clone 2A4D1 (Cat # MAB21407) (Green). Red: Negative Control. -
Gene Info — SIGLEC1
Entrez GeneID
6614Protein Accession#
Q9BZZ2Gene Name
SIGLEC1
Gene Alias
CD169, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, SIGLEC-1, SN, dJ1009E24.1
Gene Description
sialic acid binding Ig-like lectin 1, sialoadhesin
Omim ID
600751Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000030124|sialic acid-binding immunoglobulin-like lectin 1|sialoadhesin
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Interactome
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Pathway
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