FZD4 monoclonal antibody, clone 2F3A7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: FZD4-hIgGFc transfected HEK293 cell lysates with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human FZD4.
Immunogen
Recombinant protein corresponding to amino acids 37-222 of human FZD4.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: FZD4-hIgGFc transfected HEK293 cell lysates with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396).Flow Cytometry
Flow cytometric analysis of HL-60 cells with FZD4 monoclonal antibody, clone 2F3A7 (Cat # MAB21396) (Green). Red: Negative Control. -
Gene Info — FZD4
Entrez GeneID
8322Protein Accession#
Q9ULV1Gene Name
FZD4
Gene Alias
CD344, EVR1, FEVR, FZD4S, Fz-4, FzE4, GPCR, MGC34390
Gene Description
frizzled homolog 4 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq
Other Designations
WNT receptor frizzled-4|exudative vitreoretinopathy 1 (autosomal dominant; Criswick-Schepens syndrome)|frizzled 4
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Interactome
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Pathway
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Disease
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