ITGA5 monoclonal antibody, clone 8D1G12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ITGA5-hIgGFc transfected HEK293 cell lysates with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ITGA5.
Immunogen
Recombinant protein corresponding to amino acids 111-253 of human ITGA5.
Host
Mouse
Theoretical MW (kDa)
114.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ITGA5-hIgGFc transfected HEK293 cell lysates with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390).Flow Cytometry
Flow cytometric analysis of HL-60 cells with ITGA5 monoclonal antibody, clone 8D1G12 (Cat # MAB21390) (Green). Red: Negative Control. -
Gene Info — ITGA5
Entrez GeneID
3678Protein Accession#
P08648Gene Name
ITGA5
Gene Alias
CD49e, FNRA, VLA5A
Gene Description
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Omim ID
135620Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq
Other Designations
fibronectin receptor, alpha subunit|integrin alpha 5|very late activation protein 5, alpha subunit
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Interactome
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Pathway
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Disease
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